Real-time PCR analysis was employed to measure the expression of GIMAP 1 for comparison with the protein level analysis. It was also used to assess the expression of GIMAP 1 protein after infection and/or immunization of mice with P. Methods A monoclonal antibody against mouse GIMAP 1 was developed and was used to analyse the expression of the endogenous protein in tissues of normal mice and in defined sub-populations of cells prepared from lymphoid tissues using flow cytometry. GIMAP 1 was the first reported member of this gene family, identified as a gene up-regulated at the RNA level in the spleens of mice infected with the malarial parasite, Plasmodium chabaudi. Expression of GIMAP 1, a GTPase of the immunity-associated protein family, is not up-regulated in malariaĭirectory of Open Access Journals (Sweden)įull Text Available Abstract Background GIMAP (GTPase of the immunity-associated protein family proteins are a family of putative GTPases believed to be regulators of cell death in lymphomyeloid cells.
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